Zemlińska Barbara, Sochanik Aleksander, Missol-Kolka Ewa, Szala Stanisław
Department of Molecular Biology, Center of Oncology-Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland.
Acta Biochim Pol. 2002;49(1):285-90.
We compared the efficiency of in vitro DNA transfer into selected tumor and endothelial cell lines using complexes of plasmid DNA and cationic carriers: DDAB/DOPE, DC-Chol/DOPE, Arg-Chol/DOPE, Gly-Chol/DOPE, Arg-Gly-Chol/DOPE, BGTC/DOPE, and PEI. The best carriers for transfecting the majority of tested cells lines at optimized carrier-to-DNA weight ratios were PEI and BGTC/DOPE.
我们使用质粒DNA与阳离子载体的复合物,即二甲基双十八烷基溴化铵/二油酰磷脂酰乙醇胺(DDAB/DOPE)、二油酰基胆固醇/二油酰磷脂酰乙醇胺(DC-Chol/DOPE)、精氨酸胆固醇/二油酰磷脂酰乙醇胺(Arg-Chol/DOPE)、甘氨酸胆固醇/二油酰磷脂酰乙醇胺(Gly-Chol/DOPE)、精氨酸-甘氨酸-胆固醇/二油酰磷脂酰乙醇胺(Arg-Gly-Chol/DOPE)、BGTC/DOPE和聚乙烯亚胺(PEI),比较了体外DNA导入选定肿瘤细胞系和内皮细胞系的效率。在优化的载体与DNA重量比条件下,用于转染大多数受试细胞系的最佳载体是PEI和BGTC/DOPE。