Gabdrakhmanova L A, Balaban N P, Sharipova M R, Tokmakova Iu S, Sokolova E A, Rudenskaia G N, Leshchinskaia I V
Kazan State University, ul. Kremlevskaya 18, Kazan, 420008 Russia.
Mikrobiologiia. 2002 May-Jun;71(3):323-9.
The effect of some components of cultivation medium on the growth of the streptomycin-resistant Bacillus intermedius strain 3-19 and on the production of glutamyl endopeptidase was investigated using factorial experimental design, which allowed the concentrations of peptone and inorganic phosphate to be optimized for the maximum production of the enzyme. Experiments with different peptones and casamino acids showed that the enzyme production is maximum with peptone 3 of plant origin. The addition of casamino acids or amino acids to the peptone-containing cultivation medium inhibited the production of glutamyl endopeptidase.
采用析因实验设计研究了培养基的某些成分对链霉素抗性中间芽孢杆菌3-19菌株生长及谷氨酰内肽酶产生的影响，该设计可优化蛋白胨和无机磷酸盐的浓度以实现酶的最大产量。用不同蛋白胨和酪蛋白氨基酸进行的实验表明，植物源蛋白胨3的酶产量最高。向含蛋白胨的培养基中添加酪蛋白氨基酸或氨基酸会抑制谷氨酰内肽酶的产生。
