Klein R, Baranyi U, Rössler N, Greineder B, Scholz H, Witte A
Institute of Microbiology and Genetics, University of Vienna, Austria.
Mol Microbiol. 2002 Aug;45(3):851-63. doi: 10.1046/j.1365-2958.2002.03064.x.
The double-stranded (ds)DNA virus phiCh1 infects the haloalkaliphilic archaeon Natrialba magadii. The complete DNA sequence of 58 498 bp of the temperate virus was established, and the probable functions of 21 of 98 phiCh1-encoded open reading frames (ORFs) have been assigned. This knowledge has been used to propose functional modules each required for specific functions during virus development. The phiCh1 DNA is terminally redundant and circularly permuted and therefore appears to be packaged by the so-called headful mechanism. The presence of ORFs encoding homologues of proteins involved in plasmid replication as well as experimental evidence indicate a plasmid-mediated replication strategy of the virus. Results from nanosequencing of virion components suggest covalent cross-linking of monomers of at least one of the structural proteins during virus maturation. A comparison of the phiCh1 genome with the partly sequenced genome of Halobacterium salinarum virus phiH revealed a close relationship between the two viruses, although their host organisms live in distinct environments with respect to the different pH values required for growth.
双链（ds）DNA病毒phiCh1感染嗜盐碱古菌纳氏嗜盐碱杆菌（Natrialba magadii）。已确定了这种温和病毒58498 bp的完整DNA序列，并已确定了phiCh1编码的98个开放阅读框（ORF）中21个的可能功能。这些知识已被用于提出病毒发育过程中特定功能所需的功能模块。phiCh1 DNA是末端冗余且环状排列的，因此似乎是通过所谓的“满头部”机制进行包装的。编码参与质粒复制的蛋白质同源物的ORF的存在以及实验证据表明该病毒采用质粒介导的复制策略。病毒粒子成分的纳米测序结果表明，在病毒成熟过程中，至少一种结构蛋白的单体发生了共价交联。尽管phiCh1基因组与盐生盐杆菌病毒phiH部分测序的基因组在宿主生物体生长所需的不同pH值方面生活在不同环境中，但两者之间存在密切关系。
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