Two-dimensional protein analysis by isoelectric focusing in a multi-cell plate system.

作者信息

dos Santos Ferreira Beatriz, Branco Alan Trindade, de Queiroz Karlla Salim, de Souza Filho Gonçalo Apolinàrio

机构信息

Laboratorio de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego 2000, CEP 28015-620, Campos dos Goytacazes-RJ, Brazil.

出版信息

Protein Pept Lett. 2002 Feb;9(1):39-43. doi: 10.2174/0929866023409057.

DOI:10.2174/0929866023409057

PMID:12141922

Abstract

A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.

摘要