Das J
Biochemisches Institut, Medizinischen Fakultät, Christian-Albrechts-Universität, Kiel, Germany.
Biomed Chromatogr. 1991 Sep;5(5):221-5. doi: 10.1002/bmc.1130050509.
A rapid procedure of isoelectric focusing (IEF) of proteins in polyacrylamide rod gels (i.d., 1.1 mm; length, 7.5 cm) is described. The time required for IEF can be reduced to 0.5 h by using high voltages up to 3000 V in the presence or absence of urea in the gels. When used as the first dimension of a two-dimensional technique for IEF sodium dodecyl sulphate electrophoresis, high voltage IEF gives smaller protein spots on the second dimension gel, associated with an increase in resolution. The method has been tested by a two-dimensional separation of an eye sample of the goodeid fish Xenotoca eiseni.
本文描述了一种在聚丙烯酰胺棒状凝胶（内径1.1毫米；长度7.5厘米）中对蛋白质进行等电聚焦（IEF）的快速方法。在凝胶中存在或不存在尿素的情况下，通过使用高达3000 V的高电压，IEF所需的时间可缩短至0.5小时。当用作IEF-十二烷基硫酸钠电泳二维技术的第一维时，高压IEF在二维凝胶上产生的蛋白质斑点较小，分辨率提高。该方法已通过对古氏鱼Xenotoca eiseni的眼部样本进行二维分离进行了测试。
