Kung S D, Sakano K, Gray J C, Wildman S G
J Mol Evol. 1975 Dec 31;7(1):59-64. doi: 10.1007/BF01732179.
The polypeptide composition of Fraction I protein from Nicotiana digluta, a synthetic species which arose by chromosome doubling following the interspecific hybridization of N. glutinosa and N. tabacum, has been examined by isoelectric focusing. The composition of the protein from N. digluta, which was identical to the protein from the infertile F1 hybrid N. glutinosa x N. tabacum, showed 3 polypeptides in the large subunit and 4 polypeptides in the small subunit. The large subunit polypeptides were identical to those from N. glutinosa, the maternal parent in the original hybridization, whereas the small subunit polypeptides were a composite of the small subunit polypeptides from both N. glutinosa and N. tabacum. This analysis demonstrates how the polypeptide composition of Fraction I protein evolves during the origin of new species of Nicotiana.
对粘毛烟草（Nicotiana digluta）中I级蛋白的多肽组成进行了等电聚焦分析。粘毛烟草是一个人工合成物种，由粘毛烟草（N. glutinosa）和烟草（N. tabacum）种间杂交后染色体加倍产生。粘毛烟草的蛋白组成与不育的F1杂种粘毛烟草×烟草的蛋白相同，其大亚基中有3种多肽，小亚基中有4种多肽。大亚基多肽与原始杂交中的母本粘毛烟草的多肽相同，而小亚基多肽是粘毛烟草和烟草小亚基多肽的复合体。该分析表明了烟草新物种形成过程中I级蛋白多肽组成是如何演变的。
