Kwanyuen P, Wildman S G
Biochim Biophys Acta. 1975 Sep 9;405(1):167-74. doi: 10.1016/0005-2795(75)90327-x.
Ferredoxin was purified from 10 species of Nicotiana and spinach leaves. Fingerprints showed all to contain five major tryptic peptides. Some of the spinach peptides were different in RF and mobility from the Nicotiana peptides, but none of the Nicotiana ferredoxins had peptides which could distinguish one species of ferredoxin from another. Electrofocusing S-carbaminomethylcysteinyl ferredoxins showed spinach ferredoxin to have a more acidic and N. glutinosa ferredoxin a slightly more acidic isoelectric point than the other 9 Nicotiana species which were alike. Electro-focusing ferredoxin from the hybrid N. glutinosa female times N. glauca male resolved two bands or isozymes of ferredoxin, one corresponding to N. glutinosa, the other to N. glauca, the code for the latter having come from the DNA in the N. glauca pollen used to form the hybrid plant. N. glutinosa ferredoxin does not contain methionine and is different from N. tabacum and N. glauca ferredoxins which contain methionine. The N. glutinosa female times N. glauca male ferredoxin contained one-half the methionine found in N. glauca ferredoxin, thus confirming that some of the genetic information for ferredoxin in the hybrid was originally contained in the nuclear DNA of N. glauca.
铁氧化还原蛋白是从10种烟草属植物和菠菜叶中提纯出来的。指纹图谱显示，所有样本均含有5种主要的胰蛋白酶解肽段。菠菜的一些肽段在比移值和迁移率上与烟草的肽段不同，但没有一种烟草的铁氧化还原蛋白具有能区分不同种类铁氧化还原蛋白的肽段。对S-氨甲酰甲基半胱氨酰铁氧化还原蛋白进行等电聚焦分析表明，菠菜铁氧化还原蛋白比其他9种相似的烟草属植物的铁氧化还原蛋白具有更低的等电点，而粘毛烟草铁氧化还原蛋白的等电点略低。对粘毛烟草雌株与粉蓝烟草雄株杂交后代的铁氧化还原蛋白进行等电聚焦分析，分离出了两条铁氧化还原蛋白带或同工酶，一条对应于粘毛烟草，另一条对应于粉蓝烟草，后者的编码来自用于培育杂交植物的粉蓝烟草花粉中的DNA。粘毛烟草铁氧化还原蛋白不含甲硫氨酸，与含甲硫氨酸的烟草和粉蓝烟草铁氧化还原蛋白不同。粘毛烟草雌株与粉蓝烟草雄株杂交后代的铁氧化还原蛋白所含甲硫氨酸量是粉蓝烟草铁氧化还原蛋白的一半，从而证实了杂交后代中铁氧化还原蛋白的一些遗传信息最初包含在粉蓝烟草的核DNA中。
