野生桑蚕（Bombyx mandarina）W染色体上的嵌套反转录转座子。
Nested retrotransposons on the W chromosome of the wild silkworm Bombyx mandarina.
作者信息
Abe H, Sugasaki T, Terada T, Kanehara M, Ohbayashi F, Shimada T, Kawai S, Mita K, Oshiki T
机构信息
Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
出版信息
Insect Mol Biol. 2002 Aug;11(4):307-14. doi: 10.1046/j.1365-2583.2002.00339.x.
The W chromosome of the silkworms Bombyx mori or B. mandarina is recombinationally isolated from the Z chromosome and the autosomes. We previously characterized a female-specific randomly amplified polymorphic DNA (RAPD), designated W-Yamato, derived from the W chromosome of the wild silkworm Bombyx mandarina. To further analyse the W chromosome of B. mandarina, we obtained a lambda phage clone that contains the W-Yamato RAPD sequence and sequenced the 16.7 kb DNA insert. We found that this DNA comprises a nested structure of at least seven elements: six retrotransposons and one transposable element-like sequence. The transposable element-like sequence is inserted into a micropia-like retrotransposon (Karate). The Karate and the non-long terminal repeat (non-LTR) retrotransposon BMC1 are inserted into a 412-like retrotransposon (Judo). Furthermore, this Judo, and two non-LTR retrotransposons (Kurosawa and Kendo) are inserted into a Pao-like retrotransposon (Yamato). These results indicate that the retrotransposons inserted into the W chromosome are not efficiently removed but accumulate gradually as strata without recombination.
家蚕（Bombyx mori）或野桑蚕（B. mandarina）的W染色体在重组上与Z染色体和常染色体分离。我们之前鉴定了一种源自野桑蚕W染色体的雌性特异性随机扩增多态性DNA（RAPD），命名为W - Yamato。为了进一步分析野桑蚕的W染色体，我们获得了一个包含W - Yamato RAPD序列的λ噬菌体克隆，并对16.7 kb的DNA插入片段进行了测序。我们发现该DNA包含至少七个元件的嵌套结构：六个逆转录转座子和一个类转座元件序列。类转座元件序列插入到一个类似micropia的逆转录转座子（Karate）中。Karate和非长末端重复（non - LTR）逆转录转座子BMC1插入到一个类似412的逆转录转座子（Judo）中。此外，这个Judo以及两个非LTR逆转录转座子（Kurosawa和Kendo）插入到一个类似Pao的逆转录转座子（Yamato）中。这些结果表明，插入到W染色体中的逆转录转座子没有被有效去除，而是作为无重组的层次逐渐积累。
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