在家蚕（Bombyx mori）和野桑蚕（B. mandarina）的W染色体上鉴定新型随机扩增多态性DNA（RAPD）及其逆转座子相关核苷酸序列。

Identification of novel random amplified polymorphic DNAs (RAPDs) on the W chromosome of the domesticated silkworm, Bombyx mori, and the wild silkworm, B. mandarina, and their retrotransposable element-related nucleotide sequences.

作者信息

Abe H, Kanehara M, Terada T, Ohbayashi F, Shimada T, Kawai S, Suzuki M, Sugasaki T, Oshiki T

机构信息

Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.

出版信息

Genes Genet Syst. 1998 Aug;73(4):243-54. doi: 10.1266/ggs.73.243.

DOI:10.1266/ggs.73.243

PMID:9880922

Abstract

Genomic DNAs were compared between males and females of the domesticated silkworm, Bombyx mori, strains C108, C137, J137, p50, and WILD-W (constructed by crossing a wild silkworm, B. mandarina, female with a male of strain C108) by polymerase chain reaction (PCR) with 700 arbitrary 10-mer primers. Four female-specific RAPDs (W-Kabuki, W-Samurai, W-Kamikaze, and W-Yamato) were found. The sex chromosome formulas of B. mori and B. mandarina are ZW (XY) for the female and ZZ (XX) for the male. The four female-specific RAPDs are assumed to be derived from the W chromosome because the other chromosomes are shared by both sexes. A computer search for deduced amino acid sequences of these four RAPDs revealed that all of them showed homology to previously reported amino acid sequences encoded in known retrotransposable elements from various organisms.

摘要

利用700条随机10聚体引物，通过聚合酶链反应（PCR），比较了家蚕C108、C137、J137、p50和WILD-W（通过将一只野生蚕（野桑蚕）雌性与C108品系雄性杂交构建）品系的雌雄基因组DNA。发现了四个雌性特异性随机扩增多态性DNA（W-歌舞伎、W-武士、W-神风、W-大和）。家蚕和野桑蚕的性染色体组成，雌性为ZW（XY），雄性为ZZ（XX）。这四个雌性特异性随机扩增多态性DNA被认为来源于W染色体，因为其他染色体是两性共有的。对这四个随机扩增多态性DNA推导的氨基酸序列进行计算机搜索，结果显示它们都与先前报道的来自各种生物的已知逆转录转座元件编码的氨基酸序列具有同源性。