Merkle P, Bindewald H, Breitig D
Langenbecks Arch Chir. 1975 Sep 10;338(4):291-7. doi: 10.1007/BF01261718.
In this study we investigated the function of the obstructed small bowel of the rat, the behaviour of the mucosal enzymes, the metabolic changes of the small bowel wall and the morphology of the mucosa. We found a decrease of passive transport of 3H-Antipyrine which was equal after 24 and 48 hrs. The active transport of 14C-Glucose was found to be progressively inhibited after occlusion. The metabolic enzymes SDH, G-6-PDH, and GOT remained unchanged, LDH was increased after 48 hrs, which can be explained by enzyme induction. The lactate-pyruvate ratio in the tissue of the obstructed bowel was 3 times as high as in the controls. The brush-border enzymes maltase and especially the alkaline phosphatase are decreased with progressive obstruction, which probably is caused by diffusion into the lumen. By electron-microscopy there are no changes in the brush-border membrane but a swelling of mitochondria which is caused by hypoxia.
在本研究中，我们调查了大鼠梗阻小肠的功能、黏膜酶的行为、小肠壁的代谢变化以及黏膜的形态。我们发现3H-安替比林的被动转运在24小时和48小时后均有所下降。14C-葡萄糖的主动转运在梗阻后逐渐受到抑制。代谢酶琥珀酸脱氢酶（SDH）、葡萄糖-6-磷酸脱氢酶（G-6-PDH）和谷草转氨酶（GOT）保持不变，乳酸脱氢酶（LDH）在48小时后升高，这可通过酶诱导来解释。梗阻肠段组织中的乳酸-丙酮酸比值是对照组的3倍。随着梗阻进展，刷状缘酶麦芽糖酶尤其是碱性磷酸酶减少，这可能是由于向肠腔扩散所致。通过电子显微镜观察，刷状缘膜没有变化，但线粒体肿胀，这是由缺氧引起的。
