Afify Alaa M, al-Khafaji Basim M
Department of Pathology, University of Michigan Medical School, 1500 East Medical Center Drive, 2G322, Box 0054, Ann Arbor, Michigan 48109, USA.
Acta Cytol. 2002 Jul-Aug;46(4):675-8. doi: 10.1159/000326974.
To evaluate the diagnostic utility of the immunohistochemical expression of thyroid transcription factor-1 (TTF-1) in adenocarcinomas from serous fluid specimens.
Archival paraffin-embedded cell blocks of serous fluids from 82 cases, including 34 cases of metastatic lung adenocarcinoma, 12 of metastatic ovarian adenocarcinoma, 12 of metastatic breast adenocarcinoma, 12 of metastatic gastrointestinal adenocarcinoma and 12 of malignant mesothelioma, were immunostained with anti-TTF-1. All the staining was carried out using a Ventana Automated System. Staining was evaluated according to the intensity of the nuclear staining (1+ to 4+) by two observers.
Of the metastatic lung adenocarcinomas, 79% (27/34) expressed 3+ to 4+ reactivity against TTF-1. None of the malignant mesotheliomas or other metastatic adenocarcinomas expressed nuclear reactivity.
Immunostaining with TTF-1 is a useful marker that can be applied to cytologic specimens. Anti-TTF-1 can be used as a reliable component of an antibody panel to support the diagnosis of adenocarcinoma of pulmonary origin in patients presenting with metastatic adenocarcinoma in serous fluid with an unknown primary site.
评估甲状腺转录因子-1（TTF-1）免疫组化表达在浆液性液体标本腺癌诊断中的应用价值。
对82例浆液性液体的存档石蜡包埋细胞块进行抗TTF-1免疫染色，其中包括34例转移性肺腺癌、12例转移性卵巢腺癌、12例转移性乳腺腺癌、12例转移性胃肠道腺癌和12例恶性间皮瘤。所有染色均使用Ventana自动系统进行。由两名观察者根据核染色强度（1+至4+）对染色进行评估。
在转移性肺腺癌中，79%（27/34）对TTF-1表现出3+至4+的反应性。恶性间皮瘤或其他转移性腺癌均未表现出核反应性。
TTF-1免疫染色是一种可应用于细胞学标本的有用标志物。抗TTF-1可作为抗体组合的可靠组成部分，用于支持诊断原发性部位不明的浆液性液体转移性腺癌患者的肺源性腺癌。
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