Bosch Thomas C G, Augustin René, Gellner Klaus, Khalturin Konstantin, Lohmann Jan U
Zoological Institute, Christian-Albrechts-University, Olshausenstrasse, Kiel, Germany.
Differentiation. 2002 Jun;70(4-5):140-7. doi: 10.1046/j.1432-0436.2002.700403.x.
In vivo electroporation is used to study gene regulation and gene function in the freshwater polyp Hydra. Although this approach has been used successfully by several investigators, efficacy and handling continue to present a problem. Here we show technical aspects of in vivo electroporation for introducing fluorescent dyes, plasmid DNA and double stranded RNA into Hydra polyps. We describe the fundamentals of the electroporation delivery system, discuss recent studies where this approach has been used successfully, compare it to alternative transfection methods such as lipofection, and identify future directions.
体内电穿孔法用于研究淡水水螅的基因调控和基因功能。尽管已有几位研究者成功使用了这种方法，但效率和操作仍存在问题。在此，我们展示了将荧光染料、质粒DNA和双链RNA导入水螅体内的体内电穿孔技术细节。我们描述了电穿孔递送系统的基本原理，讨论了最近成功使用该方法的研究，将其与脂质转染等其他转染方法进行了比较，并确定了未来的发展方向。
