Gou Ke-Mian, An Xiao-Rong, Tian Jian-Hui, Chen Yong-Fu
National Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China.
Sheng Wu Gong Cheng Xue Bao. 2002 Jan;18(2):144-8.
The production of human recombinant proteins in milk of transgenic farm animals offers a safe, very cost-effective source of commercially important proteins that cannot be produced as efficiently in adequate quantities by other methods. This review has summarized the current status of gene selection, vector construct, transgenic methods, economics, and obvious potential in transgenic animals bioreactors. Recently, a more powerful approach was adopted in the transgenic animals founded on the application of nuclear transfer. As we will illustrate, this strategy presents a breakthrough in the overall efficiency of generating transgenic farm animals, product consistency, and time of product development. The successful adaptation of Cre-/lox P-mediated site-specific DNA recombination systems in farm animals will offer unprecedented possibilities for generating transgenic animals.
利用转基因农场动物的乳汁生产人重组蛋白，为商业上重要的蛋白质提供了一种安全且极具成本效益的来源，而其他方法无法如此高效且足量地生产这些蛋白质。本综述总结了转基因动物生物反应器在基因选择、载体构建、转基因方法、经济性及明显潜力方面的现状。最近，基于核移植技术在转基因动物中采用了一种更强大的方法。正如我们将阐述的，该策略在转基因农场动物的整体生产效率、产品一致性及产品开发时间方面带来了突破。Cre-/lox P介导的位点特异性DNA重组系统在农场动物中的成功应用，将为转基因动物的培育带来前所未有的可能性。
