Altindis Mustafa, Dilek Osman Nuri, Demir Serap, Akbulut Gorhan
Kocatepe University, School of Medicine, Departments of Microbiology and Clinical Microbiology, Afyon, Turkey.
Acta Gastroenterol Belg. 2002 Apr-Jun;65(2):74-6.
Several diagnostic tests are available for evaluating Helicobacter pylori (H. pylori) infection: histological examination, culture of gastric biopsy specimens, rapid urease test, urea breath test and serology. In this study, we assessed the reliability of a newly developed enzyme immunoassay HpSA (H. pylori Stool Antigen) kit for detecting H. pylori antigen in stool. Eighty-five patients (50 males, 35 females; mean age 41.6 +/- 9.8 years) with dyspeptic symptoms who were examined by upper gastrointestinal endoscopy. The patients with a history of previous treatment with proton pump inhibitors, bismuth compounds or antibiotics were excluded. During the endoscopic examination biopsies were taken from antrum and corpus for rapid urease test and histological examination. Stool specimens were submitted to the laboratory and HpSA test was performed. H. pylori was considered in condition with rapid urease test and histopathological examination for H. pylori positive. Forty-six of 85 patients were positive and remaining 39 patients were negative for H. pylori with the rapid urease test and pathologic evaluation. When 0.160 was adopted as the cut-off value, in accordance with the manufacturer's recommendations; stool antigen has been detected in 45 of the 46 H. pylori positive patients. The sensitivity and specificity of HpSA test were 97.8%, 94.9% respectively. These results indicate that HpSA is a highly reliable diagnostic method for H. pylori infection.
有几种诊断测试可用于评估幽门螺杆菌（H. pylori）感染：组织学检查、胃活检标本培养、快速尿素酶试验、尿素呼气试验和血清学检测。在本研究中，我们评估了一种新开发的酶免疫测定HpSA（幽门螺杆菌粪便抗原）试剂盒检测粪便中幽门螺杆菌抗原的可靠性。85例有消化不良症状的患者（50例男性，35例女性；平均年龄41.6±9.8岁）接受了上消化道内镜检查。排除有质子泵抑制剂、铋剂或抗生素治疗史的患者。在内镜检查期间，从胃窦和胃体取活检组织进行快速尿素酶试验和组织学检查。粪便标本送检实验室并进行HpSA检测。快速尿素酶试验和组织病理学检查结果均为幽门螺杆菌阳性者判定为感染幽门螺杆菌。85例患者中，46例快速尿素酶试验和病理评估显示幽门螺杆菌阳性，其余39例为阴性。按照制造商的建议，当采用0.160作为临界值时，46例幽门螺杆菌阳性患者中有45例检测到粪便抗原。HpSA检测的敏感性和特异性分别为97.8%、94.9%。这些结果表明，HpSA是一种检测幽门螺杆菌感染高度可靠的诊断方法。
