Westphal G, van den Berg-Stein S, Braun K, Knoch T A, Dümmerling M, Langowski J, Debus J, Friedrich E
Division Radiobiology in Radiooncology, German Cancer Research Center, DKFZ, Heidelberg, Germany.
J Exp Clin Cancer Res. 2002 Jun;21(2):255-67.
The receptors for nerve growth factor (NGF)--TrkA and p75NTR--were detected at the mRNA and the protein level in various human tumor cell lines. The NGF receptor TrkA was found on all examined tumor cell lines and is not restricted to cells belonging to the nervous system. NGF did not influence the proliferation rate of TrkA-positive cells NMB, K562, UT-7/EPO and PC-12. After NGF induction, the production level of the differentiation marker c-fos was increased in UT-7/EPO and PC- 12 cells. NGF-treatment of the UT-7/EPO cells and deprivation of erythropoietin (EPO) led to the new adherent cell line UT-7/NGF. Although UT-7/NGF showed a similar growth curve as UT-7/EPO, there were differences in the pattern of adhesion molecules and of the cytoskeleton. The effect of NGF on the cytoskeleton could not be induced in other human cell lines like NMB or KTCTL-30. TrkA inhibition with K252a--a blocker of Trk-induced receptor kinase--suggests, that the NGF signal may be transduced by the TrkA receptor in UT-7/NGF cells. This indicates that NGF is a decisive mediator of cellular adhesion.
在多种人类肿瘤细胞系中，通过mRNA和蛋白质水平检测到了神经生长因子（NGF）的受体——TrkA和p75NTR。在所有检测的肿瘤细胞系中均发现了NGF受体TrkA，且不仅限于属于神经系统的细胞。NGF不影响TrkA阳性细胞NMB、K562、UT-7/EPO和PC-12的增殖率。在NGF诱导后，UT-7/EPO和PC-12细胞中分化标志物c-fos的产生水平增加。对UT-7/EPO细胞进行NGF处理并剥夺促红细胞生成素（EPO），产生了新的贴壁细胞系UT-7/NGF。尽管UT-7/NGF显示出与UT-7/EPO相似的生长曲线，但在黏附分子模式和细胞骨架方面存在差异。在其他人类细胞系如NMB或KTCTL-30中，NGF对细胞骨架的作用无法被诱导。用Trk诱导的受体激酶阻滞剂K252a抑制TrkA表明，NGF信号可能在UT-7/NGF细胞中由TrkA受体转导。这表明NGF是细胞黏附的决定性介质。
