WC1富含半胱氨酸的清道夫受体结构域9和11是WC1反受体的受体。

Scavenger receptor cysteine-rich domains 9 and 11 of WC1 are receptors for the WC1 counter receptor.

作者信息

Ahn J S, Konno A, Gebe J A, Aruffo A, Hamilton M J, Park Y H, Davis W C

机构信息

Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.

出版信息

J Leukoc Biol. 2002 Aug;72(2):382-90.

DOI:

PMID:12149430

Abstract

Workshop cluster 1 (WC1) is a member of the scavenger receptor cysteine-rich (SRCR) superfamily that includes CD5, CD6, CD163, and M160. Bovine WC1 consists of 11 SRCR domains, a unique domain 1, and two homologous 5 SRCR domain cassettes, WC1 domains 2-6 and 7-11. The porcine orthologue of WC1 contains five SRCR domains with a different domain arrangement. Although the function of WC1 is unknown, WC1 is proposed to be an accessory or homing molecule. Thus, identification of cells that express the counter receptor for WC1 (WC1-CR) is critical to understanding the function of WC1. For this reason, we constructed WC1-human immunoglobulin G1 fusion proteins to identify the binding domain of WC1 and cells that express the WC1-CR. Immunohistochemical analysis revealed WC1 domains 9 and 11 bind cells with macrophage and dendritic cell morphology and cells in ellipsoids in the spleen. These results and the finding of conserved signaling motifs in the cytoplasmic tail suggest WC1 may be an accessory molecule.

摘要

研讨会集群1（WC1）是富含半胱氨酸的清道夫受体（SRCR）超家族的成员，该超家族包括CD5、CD6、CD163和M160。牛WC1由11个SRCR结构域、一个独特的结构域1以及两个同源的5个SRCR结构域盒，即WC1结构域2 - 6和7 - 11组成。猪WC1的直系同源物包含五个SRCR结构域，其结构域排列不同。尽管WC1的功能尚不清楚，但有人提出WC1是一种辅助或归巢分子。因此，鉴定表达WC1的反受体（WC1 - CR）的细胞对于理解WC1的功能至关重要。出于这个原因，我们构建了WC1 - 人免疫球蛋白G1融合蛋白，以鉴定WC1的结合结构域和表达WC1 - CR的细胞。免疫组织化学分析显示，WC1结构域9和11与具有巨噬细胞和树突状细胞形态的细胞以及脾脏椭球体中的细胞结合。这些结果以及在细胞质尾部发现的保守信号基序表明WC1可能是一种辅助分子。