Chen Xiuxu, Lai Mao-de, Huang Qiong
Department of Pathology, School of Medicine, Zhejiang University, Hangzhou 310031, China.
Chin Med J (Engl). 2002 Jul;115(7):1048-52.
To study the relationship between sensitivity to 5-FU and the status of a panel of microsatellite loci in three human colon cancer cell lines.
Cell viability in several concentrations of 5-FU was assessed by the MTT test. Expression of hMSH2 and hMLH1 in LoVo, SW480 and SW1116 cells were analyzed by immunocytochemical staining.Ten mononucleotide and dinucleotide microsatellite loci were analyzed by the PCR-SSLP-silver staining method.
By MTT assay, it showed that LoVo cells were more sensitive than SW480 and SW1116 cells (0.8 micromol/L,2.2 micromol/L and 1.9 micromol/L, respectively, P < 0.05). By immunocytochemical staining, hMSH2 was expressed in SW480 and SW1116 cells but not in LoVo cells, while hMLH1 was positive in all three cell lines. The PCR-SSLP-silver staining of 10 microsatellite loci revealed that LoVo cells had a different pattern of electrophoretic bands compared with SW480 and SW1116 cells, manifesting both additions and band-shifts.
Together with hMSH2 and hMLH1, the status of a panel of microsatellite loci may be used as convenient predictors for drug-optimization or prognosis-assessment in colorectal cancer patients before chemotherapy.
研究三种人结肠癌细胞系对5-氟尿嘧啶（5-FU）的敏感性与一组微卫星位点状态之间的关系。
采用MTT试验评估几种浓度5-FU作用下的细胞活力。通过免疫细胞化学染色分析LoVo、SW480和SW1116细胞中hMSH2和hMLH1的表达。采用PCR-SSLP-银染法分析10个单核苷酸和二核苷酸微卫星位点。
MTT试验显示，LoVo细胞比SW480和SW1116细胞更敏感（分别为0.8微摩尔/升、2.2微摩尔/升和1.9微摩尔/升，P<0.05）。免疫细胞化学染色显示，hMSH2在SW480和SW1116细胞中表达，而在LoVo细胞中不表达，而hMLH1在所有三种细胞系中均呈阳性。10个微卫星位点的PCR-SSLP-银染显示，与SW480和SW1116细胞相比，LoVo细胞具有不同的电泳带模式，表现为条带增加和条带移位。
一组微卫星位点的状态连同hMSH2和hMLH1，可作为结直肠癌患者化疗前药物优化或预后评估的便捷预测指标。
