Liu Suying, Feng Youji
Obstetrics and Gynecologic Hospital of Fudan University, Shanghai 200011, China.
Chin Med J (Engl). 2002 Jul;115(7):1074-7.
To investigate the effects of sulindac metabolites on proliferation and apoptosis in the human umbilical vein endothelial cell line ECV304 in vitro.
The proliferation profile of ECV304 was determined by methyl thiazolyl tetrazolium (MTT) method. Cell cycle distribution, apoptosis and the ultrastructure of ECV304 were detected by flow cytometry (FCM) and electron microscopy, respectively.
MTT assay showed that the sulfide inhibited the proliferation of ECV304 and its effect was dose-dependent; the IC(50) was 200 micromol/L. FCM showed that the sulfide changed cell cycle distribution. The cell cycle distribution was as follows: G(1) phase (control group 77.74% +/- 1.58%; sulfone group 75.63% +/- 2.12%; sulfide group 46.12% +/- 1.60%); S phase (control group 13.64% +/- 1.22%; sulfone group 16.40 +/- 2.30%; sulfide group 27.26% +/- 2.08%); G(2)-M phase (control group 8.61% +/- 0.67%; sulfone group 7.98% +/- 0.49%; sulfide group 26.62% +/- 3.54%). The apoptosis rates in the control group, sulfone group and sulfide group were 6.08% +/- 3.39%, 4.81% +/- 2.14% and 51.90% +/- 5.67%, respectively. Sulfide reduced the proportion of G(1) phase, increased the proportion of S phase, G(2)-M phase and the apoptosis rate significantly (P < 0.01, vs control). In the sulfide-treated cells, there were nuclear fragmentation and chromosomal condensation, shrinkage of the cell and loss of contact with neighboring cells. Apoptotic bodies were observed. Sulfone showed no effect on cell proliferation, cell cycle distribution or cell morphology.
Sulfide can significantly reduce the proliferation of ECV304, change the cell cycle distribution and arrest cells in G(2)-M phase where apoptosis may be induced. Sulfone has no such effects on this cell line.
研究舒林酸代谢产物对人脐静脉内皮细胞系ECV304体外增殖和凋亡的影响。
采用噻唑蓝（MTT）法测定ECV304的增殖情况。分别通过流式细胞术（FCM）和电子显微镜检测ECV304的细胞周期分布、凋亡情况及超微结构。
MTT分析显示，硫化物抑制ECV304的增殖，且其作用呈剂量依赖性；半数抑制浓度（IC50）为200 μmol/L。FCM显示，硫化物改变细胞周期分布。细胞周期分布情况如下：G1期（对照组77.74%±1.58%；砜类组75.63%±2.12%；硫化物组46.12%±1.60%）；S期（对照组13.64%±1.22%；砜类组16.40±2.30%；硫化物组27.26%±2.08%）；G2-M期（对照组8.61%±0.67%；砜类组7.98%±0.49%；硫化物组26.62%±3.54%）。对照组、砜类组和硫化物组的凋亡率分别为6.08%±3.39%、4.81%±2.14%和51.90%±5.67%。硫化物显著降低G1期比例，增加S期、G2-M期比例及凋亡率（与对照组相比，P<0.01）。在经硫化物处理的细胞中，可见核碎裂、染色体凝聚、细胞皱缩及与相邻细胞失去接触。观察到凋亡小体。砜类对细胞增殖、细胞周期分布或细胞形态无影响。
硫化物可显著降低ECV304的增殖，改变细胞周期分布并使细胞停滞于G2-M期，在此期可能诱导细胞凋亡。砜类对该细胞系无此作用。
