通过限制性内切酶位点基因组扫描对癌症中异常甲基化的研究。

The study of aberrant methylation in cancer via restriction landmark genomic scanning.

作者信息

Smiraglia Dominic J, Plass Christoph

机构信息

Division of Human Cancer Genetics, Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University, Columbus, Ohio, OH 43210, USA.

出版信息

Oncogene. 2002 Aug 12;21(35):5414-26. doi: 10.1038/sj.onc.1205608.

DOI:10.1038/sj.onc.1205608

PMID:12154404

Abstract

Restriction landmark genomic scanning (RLGS) has been used to study DNA methylation in cancer for nearly a decade. The strong bias of RLGS for assessing the methylation state of CpG islands genome wide makes this an attractive technique to study both hypo- and hypermethylation of regions of the genome likely to harbor genes. RLGS has been used successfully to identify regions of hypomethylation, candidate tumor suppressor genes, correlations between hypermethylation events and clinical factors, and quantification of hypermethylation in a multitude of malignancies. This review will examine the major uses of RLGS in the study of aberrant methylation in cancer and discuss the significance of some of the findings.

摘要

限制酶切位点基因组扫描（RLGS）已被用于癌症DNA甲基化研究近十年。RLGS在全基因组范围内评估CpG岛甲基化状态时具有很强的偏向性，这使得它成为研究基因组中可能包含基因区域的低甲基化和高甲基化的一种有吸引力的技术。RLGS已成功用于识别低甲基化区域、候选肿瘤抑制基因、高甲基化事件与临床因素之间的相关性，以及多种恶性肿瘤中高甲基化的定量分析。本文将探讨RLGS在癌症异常甲基化研究中的主要用途，并讨论一些研究结果的意义。