对19.5 kDa抗原的血清反应性与对35 kDa抗原缺乏血清反应性相结合，与胃腺癌风险增加相关。

Seroreactivity to 19.5-kDa antigen in combination with absence of seroreactivity to 35-kDa antigen is associated with an increased risk of gastric adenocarcinoma.

作者信息

Chua Tju Siang, Fock Kwong Ming, Chan Yiong Huak, Dhamodaran Subbiah, Sim Chee Seng, Ng Tay Meng, Teo Eng Kiong

机构信息

Division of Gastroenterology, Department of Medicine, Changi General Hospital, Singapore.

出版信息

Helicobacter. 2002 Aug;7(4):257-64. doi: 10.1046/j.1523-5378.2002.00089.x.

DOI:10.1046/j.1523-5378.2002.00089.x

PMID:12165034

Abstract

BACKGROUND

Only a minority of those infected with Helicobacter pylori will develop gastric cancer. Stratification of H. pylori strains based on carcinogenic potential will provide a basis for selective surveillance and eradication therapy. We studied the anti-H. pylori antibody profile in Asian patients with gastric adenocarcinoma to identify any H. pylori antigen that may be associated with an increased or decreased risk of gastric carcinoma.

PATIENTS AND METHODS

A case-control study comparing the seroprevalence of antibodies with various H. pylori antigens in Singaporeans with gastric adenocarcinoma and the normal Singaporean population was carried out using both conventional immunoglobulin (Ig) G enzyme-linked immunosorbent assay (ELISA) and Western blot immunoassay.

RESULTS

The seroprevalence among 44 gastric adenocarcinoma cases (70.5% males, mean age 66.7 +/- 13.5 years) and 261 controls (49.8% males, mean age 61.5 +/- 4.1 years) was 90.9% vs. 50.2% by IgG ELISA. In the H. pylori-positive male population, those suffering from gastric adenocarcinoma had significantly lower seroreactivity to the 35-kDa antigen compared with asymptomatic controls (p =.0198, OR = 3.79, 95% CI 1.24-11.61). Seropositivity to the 19.5 kDa antigen was also found to be associated with the presence of gastric adenocarcinoma in Singaporean males (p =.022, OR = 4.17, 95% CI 1.22-14.28). A 'high-risk' phenotype consisting of absence of a band at 35-kDa in combination with the presence of a band at 19.5-kDa was significantly associated with the presence of gastric adenocarcinoma (p =.002, OR = 3.7, 95% CI 1.6-8.6).

CONCLUSIONS

Stratification of H. pylori strains based on their potential for carcinogenesis, such as those strains that are seropositive for the 19.5 kDa antigen and seronegative for the 35-kDa antigen, may provide a basis for selective eradication of H. pylori infection and future vaccine development.

摘要

背景

幽门螺杆菌感染者中仅有少数会发展为胃癌。根据致癌潜力对幽门螺杆菌菌株进行分层可为选择性监测和根除治疗提供依据。我们研究了亚洲胃腺癌患者的抗幽门螺杆菌抗体谱，以确定可能与胃癌风险增加或降低相关的任何幽门螺杆菌抗原。

患者与方法

采用传统免疫球蛋白（Ig）G酶联免疫吸附测定（ELISA）和蛋白质印迹免疫测定法，对新加坡胃腺癌患者和正常新加坡人群中针对各种幽门螺杆菌抗原的抗体血清阳性率进行病例对照研究。

结果

44例胃腺癌患者（男性占70.5%，平均年龄66.7±13.5岁）和261例对照者（男性占49.8%，平均年龄61.5±4.1岁）中，IgG ELISA检测的血清阳性率分别为90.9%和50.2%。在幽门螺杆菌阳性男性人群中，胃腺癌患者对35 kDa抗原的血清反应性显著低于无症状对照者（p = 0.0198，OR = 3.79，95% CI 1.24 - 11.61）。还发现对19.5 kDa抗原的血清阳性与新加坡男性胃腺癌的存在有关（p = 0.022，OR = 4.17，95% CI 1.22 - 14.28）。由35 kDa处无条带与19.5 kDa处有条带组成的“高危”表型与胃腺癌的存在显著相关（p = 0.002，OR = 3.7，95% CI 1.6 - 8.6）。