Faridah M N, Shahrom A W
Department of Pathology, Faculty of Medicine, Hospital Universiti Kebangsaan Malaysia, Kuala Lumpur.
Malays J Pathol. 2001 Dec;23(2):111-4.
This paper describes a modified method of quantitative determination of histamine in human skin wounds using fluorescence spectrophotometer. In this study, histamine was used as an indicator to differentiate antemortem from postmortem wounds. Skin samples were obtained from 20 corpses which were brought to Hospital Kuala Lumpur and Universiti Kebangsaan Malaysia for medicolegal autopsy. Sections of human skin were processed biochemically for histamine determination using fluorescence spectrophotometer. Results revealed no significant difference in the histamine content of the antemortem wounds in comparison to postmortem wounds. Based on these results, detection of histamine is not suitable to differentiate antemortem from postmortem wounds.
本文描述了一种使用荧光分光光度计定量测定人皮肤伤口中组胺的改良方法。在本研究中，组胺被用作区分生前伤口和死后伤口的指标。皮肤样本取自20具尸体，这些尸体被送往吉隆坡医院和马来西亚国民大学进行法医尸检。使用荧光分光光度计对人体皮肤切片进行生化处理以测定组胺。结果显示，生前伤口的组胺含量与死后伤口相比无显著差异。基于这些结果，检测组胺并不适合用于区分生前伤口和死后伤口。
