Freisleben Achim, Schieberle Peter, Rychlik Michael
Institut für Lebensmittelchemie der Technischen Universität München, Lichtenbergstrasse 4, D-85748 Garching, Germany.
J Agric Food Chem. 2002 Aug 14;50(17):4760-8. doi: 10.1021/jf025571k.
[2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.
[2H4]叶酸是通过对氨基苯甲酸进行氘代合成的，然后将其与谷氨酸和6-甲酰基蝶呤偶联。以[2H4]叶酸作为起始成分能够制备标记的维生素形式的四氢叶酸、5-甲酰基四氢叶酸、5-甲基四氢叶酸和10-甲酰基叶酸，这些通过电喷雾质谱法和碰撞诱导解离进行了表征。质谱研究证实这些化合物可用作稳定同位素稀释分析中的内标。
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