心脏流出液中的过氧化物酶活性：评估实验性缺血再灌注损伤的生化参数。
Peroxidatic activity in heart effluent: a biochemical parameter for the assessment of experimental ischemia-reperfusion injury.
作者信息
Leabu M. V., Bucur Elisa A.
机构信息
"Carol Davila" University of Medicine and Pharmacy, Faculty of Medicine, Department of Cell Biology and Histology, 8 Eroilor Sanitari Blvd., 76241 Bucharest, Romania.
出版信息
J Cell Mol Med. 2000 Jul-Sep;4(3):176-182. doi: 10.1111/j.1582-4934.2000.tb00115.x.
Peroxidatic activity in heart effluent was defined as a new biochemical parameter for the experimental study of myocardial ischemia. The peroxidatic reaction was determined by dot blot analysis with 3,3'-diaminobenzidine as hydrogen donor. After ischemia, the level of peroxidatic activity in heart effluent was 2-3 times higher than before. The effects in experimental modulation of ischemia, such as nicorandil or aprikalim protection, and the reversibility of protection by glibenclamide, could accurately be noted using the level of peroxidatic activity in heart effluent as a biochemical parameter. The results were in good agreement with those obtained for other enzymes used as biochemical parameters in experimental heart ischemia-reperfusion studies.
心脏流出液中的过氧化物酶活性被定义为心肌缺血实验研究的一个新的生化参数。过氧化物酶反应通过以3,3'-二氨基联苯胺作为氢供体的斑点印迹分析来测定。缺血后，心脏流出液中的过氧化物酶活性水平比之前高出2至3倍。使用心脏流出液中的过氧化物酶活性水平作为生化参数，可以准确地观察到实验性缺血调节的效果，如尼可地尔或阿普卡林的保护作用，以及格列本脲保护作用的可逆性。结果与在实验性心脏缺血-再灌注研究中用作生化参数的其他酶所获得的结果高度一致。
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