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[健康及变应性心脏病时家兔心肌与红细胞中的转酮醇酶活性]

[Transketolase activity in rabbit myocardium and erythrocytes in health and in allergic heart diseases].

作者信息

Grosdova M D, Astakhova T A

出版信息

Vopr Med Khim. 1975 Jul-Aug;21(4):363-6.

PMID:1216755
Abstract

Transketolase activity was determined in myocardium and erythrocytes of rabbits by following formation of D-sedoheptuloso-7-phosphate (S-7-P), when the convertion of the substance was blocked in a transaldolase reaction by addition of 3-phosphoglycerol aldehyde dehydrogenase. In the transaldolase reaction consumption of the S-7-P did not exceed 5% of the amount of this compound, produced in the transketolase reaction. In allergic impairment alterations in the transketolase activity occurred simultaneously with a change in the level of plastic metabolism in myocardium. The transketolase activity was decreased when destructive processes were stimulated. But the transketolase activity was distinctly increased under the conditions, characterized by activation of protein biosynthesis.

摘要

通过在转醛醇酶反应中添加3 - 磷酸甘油醛脱氢酶来阻断物质转化,从而通过追踪D - 景天庚酮糖 - 7 - 磷酸(S - 7 - P)的形成,测定兔心肌和红细胞中的转酮醇酶活性。在转醛醇酶反应中,S - 7 - P的消耗量不超过转酮醇酶反应中产生的该化合物量的5%。在过敏性损伤中,转酮醇酶活性的改变与心肌中物质代谢水平的变化同时发生。当破坏过程受到刺激时,转酮醇酶活性降低。但在以蛋白质生物合成激活为特征的条件下,转酮醇酶活性明显增加。

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