Krasnova V G
Vopr Onkol. 1975;21(7):28-32.
The author has studied microspectrophotometrically the DNA content in cell nuclei of 30 nonirradiated and 30 irradiated with different doses squamous-cell laryngeal cancers and their regional metastases in the growth zone of histological sections and in cytological specimens (impressions). The DNA "accumulation index" and polyploidization phenomenon were found to be considerably decreased depending on radiation dosage in the group of observations with a good clinical effect. In cytological preparations (impressions) these indices were lower, that seems to be related with the fact that cells of central tumor portions would get into the impression-preparation. Kinetic changes in the DNA content and a histographic type can characterize the tissue radiosensitivity, thus allowing an objective morphological control over radiation therapy.
作者采用显微分光光度法，研究了30例未经照射及30例接受不同剂量照射的喉鳞状细胞癌及其区域转移灶的细胞核DNA含量，取材于组织学切片的生长区及细胞学标本（印片）。在具有良好临床疗效的观察组中，发现DNA“积聚指数”和多倍体化现象随辐射剂量的增加而显著降低。在细胞学制剂（印片）中，这些指数较低，这似乎与中央肿瘤部分的细胞进入印片制剂这一事实有关。DNA含量的动力学变化和组织形态学类型可表征组织的放射敏感性，从而实现对放射治疗的客观形态学控制。
