Goodman J C, Feng Y Q, Valadka A B, Bryan R J, Robertson C S
Department of Neurosurgery, Baylor College of Medicine, Houston, Texas, USA.
Acta Neurochir Suppl. 2002;81:343-5. doi: 10.1007/978-3-7091-6738-0_87.
To examine the feasibility of measuring the nitric oxide (NO) metabolites nitrate and nitrite in microdialysate samples from the human brain, microdialysis probes were placed in normal appearing cerebral cortex of severely head injured patients in the Neurosurgical Intensive Care Unit at Ben Taub General Hospital. Nitrate/nitrite analysis was performed using NO chemiluminescence. Low micromolar levels of NO metabolites were consistently and easily detected. These levels seen are comparable to levels reported in CSF but tissue tortuosity and probe recovery considerations suggest that the absolute concentrations at the probe site are probably ten fold higher. Microdialysis with measurement of nitric oxide metabolites is technically feasible and may provide valuable insights into both normal neurochemistry and neurochemical derangements in disease.
为了研究测量来自人脑的微透析样品中一氧化氮（NO）代谢产物硝酸盐和亚硝酸盐的可行性，将微透析探针置于本陶布综合医院神经外科重症监护病房中重度颅脑损伤患者外观正常的大脑皮层中。使用NO化学发光法进行硝酸盐/亚硝酸盐分析。始终能够轻松检测到低微摩尔水平的NO代谢产物。所观察到的这些水平与脑脊液中报告的水平相当，但考虑到组织曲折度和探针回收率，表明探针部位的绝对浓度可能高出十倍。测量一氧化氮代谢产物的微透析在技术上是可行的，并且可能为正常神经化学和疾病中的神经化学紊乱提供有价值的见解。
