[Nested coamplification polymerase chain reaction].

OBJECTIVE

To develop a simple and reliable method for intensifying the hybridization signals of gene chips.

METHODS

The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip.

RESULTS

With the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater.

CONCLUSION

Adding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.