5-丙炔基尿嘧啶.二氨基嘌呤：用于DNA非酶促转录的高效碱基对。

5-propynyluracil.diaminopurine: an efficient base-pair for non-enzymatic transcription of DNA.

作者信息

Chaput John C, Sinha Surajit, Switzer Christopher

机构信息

Department of Chemistry, University of California, Riverside, CA 92521, USA.

出版信息

Chem Commun (Camb). 2002 Aug 7(15):1568-9. doi: 10.1039/b204535d.

DOI:10.1039/b204535d

PMID:12170785

Abstract

The Up.D base-pair (5-propynyl uracil.diaminopurine) is found to be more effective at non-enzymatic transcription than the corresponding natural T.A pair; under non-enzymatic reaction conditions where the natural T.A base-pair fails, a DNA template bearing Up efficiently directs the incorporation of D into a product RNA strand.

摘要

研究发现，Up.D碱基对（5-丙炔基尿嘧啶.二氨基嘌呤）在非酶促转录中比相应的天然T.A碱基对更有效；在天然T.A碱基对无法起作用的非酶促反应条件下，带有Up的DNA模板能有效地指导将D掺入产物RNA链中。