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通过酿酒酵母和戴尔布有孢圆酵母之间的原生质体融合获得的属间杂种PB2生产甘油和阿拉伯糖醇。

Glycerol and arabitol production by an intergeneric hybrid, PB2, obtained by protoplast fusion between Saccharomyces cerevisiae and Torulaspora delbrueckii.

作者信息

Lucca M E, Spencer J F T, de Figueroa L I C

机构信息

Microbiología Superior, Facultad de Bioquímica, Universidad Nacional de Tucumán, Argentina.

出版信息

Appl Microbiol Biotechnol. 2002 Aug;59(4-5):472-6. doi: 10.1007/s00253-002-1025-5. Epub 2002 Jun 26.

Abstract

An intergeneric osmotolerant hybrid yeast, PB2, was used together with the parental strains to study glycerol and arabitol production in batch culture. This fusion product was previously obtained by protoplast fusion between Torulaspora delbrueckii and Saccharomyces cerevisiae. Polyols and biomass production were determined in batch culture under aerobic conditions. Under the conditions tested, using PB2 hybrid and both parental strains, the best results were obtained with the hybrid. Arabitol reached a final concentration of 70 g/l and glycerol was increased to up to 50 g/l.

摘要

一种属间耐渗透压杂交酵母PB2与亲本菌株一起用于研究分批培养中甘油和阿拉伯糖醇的产生。这种融合产物先前是通过德巴利酵母和酿酒酵母之间的原生质体融合获得的。在有氧条件下的分批培养中测定多元醇和生物量的产生。在所测试的条件下,使用PB2杂交种和两个亲本菌株,杂交种获得了最佳结果。阿拉伯糖醇的最终浓度达到70 g/l,甘油增加到50 g/l。

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