某些葡萄球菌物质的趋化作用（作者译）

[Chemotactic effects of some staphylococcal substances (author's transl)].

作者信息

Kleickmann A, Brückler J, Blobel H, Ditzrow D

出版信息

Zentralbl Bakteriol Orig A. 1975 Oct;233(2):153-62.

Abstract

Bovine leukocytes, besides rabbit leukocytes, were found to be suitable for chemotactic studies on staphylococci with the modified Boyden-method. The bovine leukocytes could be obtained more easily in larger quantities. Casein, however, had no significant chemotactic effects on bovine leukocytes. Culture supernatants of Staphylococcus aureus and S. epidermidis and the heat-killed staphylococci had cytotaxigenic and to a small degree also cytotaxic effects on the rabbit and bovine leukocytes. The extracted "straphylococcal skeletons", "clumping factor" (CF), protein A and capsular substances were cytotaxigenic. In addition, CF was moderately cytotaxic only for rabbit leukocytes. The chomotactic migration of leukocytes was markedly reduced after their treatment with leukocidin (PVL-R) in subleukocidic concentrations.

摘要

除兔白细胞外，还发现牛白细胞适用于采用改良博伊登法对葡萄球菌进行趋化性研究。牛白细胞更容易大量获取。然而，酪蛋白对牛白细胞没有显著的趋化作用。金黄色葡萄球菌和表皮葡萄球菌的培养上清液以及热杀死的葡萄球菌对兔和牛白细胞具有细胞趋化原性，且在一定程度上也具有细胞趋化作用。提取的“葡萄球菌骨架”、“凝聚因子”（CF）、蛋白A和荚膜物质具有细胞趋化原性。此外，CF仅对兔白细胞有中度趋化作用。白细胞在亚致死浓度的杀白细胞素（PVL-R）处理后，其趋化迁移明显减少。