通过酶联免疫吸附测定法对牛感染微小隐孢子虫的血清学检测及其与粪便学检测的相关性评估

Serological detection of exposure to Cryptosporidium parvum in cattle by ELISA and its evaluation in relation to coprological tests.

作者信息

Nydam Daryl V, Lindergard Gabriella, Guard Charles L, Schaaf Stephanie L, Wade Susan E, Mohammed Hussni O

机构信息

Department of Population Medicine and Diagnostic Sciences, Section of Epidemiology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.

出版信息

Parasitol Res. 2002 Sep;88(9):797-803. doi: 10.1007/s00436-002-0665-9. Epub 2002 May 9.

DOI:10.1007/s00436-002-0665-9

PMID:12172810

Abstract

We evaluated serum examination as an alternative to fecal analysis for the diagnosis of exposure to Cryptosporidium parvum in cattle. The accuracy of the serum ELISA was compared to the combined results of concentration flotation microscopy and fecal enzyme immunoassay. The expected performance of the serum ELISA at different levels of infection with C. parvum was evaluated using the predicative values positive and negative. Optimal conditions for the serum ELISA can be achieved by diluting the serum samples 1:20 and the conjugate 1:8,000. The serum ELISA had a relatively high sensitivity of 97.5% (95% CI=87-100%) and poor specificity, 4% (95% CI=1-20%). There was a poor agreement between the serum ELISA and the fecal tests (kappa=0) on samples collected from adult cows in a high-risk and a low-risk population. Examination of some of these fecal samples using a PCR detection method demonstrated the presence of C. parvum DNA in 10% of the samples.

摘要

我们评估了血清检测作为粪便分析的替代方法，用于诊断牛感染微小隐孢子虫的情况。将血清酶联免疫吸附测定（ELISA）的准确性与浓缩浮选显微镜检查和粪便酶免疫测定的综合结果进行了比较。使用阳性和阴性预测值评估了血清ELISA在不同微小隐孢子虫感染水平下的预期性能。血清ELISA的最佳条件可通过将血清样本稀释1:20和将结合物稀释1:8000来实现。血清ELISA具有相对较高的敏感性，为97.5%（95%置信区间=87-100%），但特异性较差，为4%（95%置信区间=1-20%）。在高风险和低风险人群中从成年母牛采集的样本上，血清ELISA与粪便检测之间的一致性较差（kappa=0）。使用聚合酶链反应（PCR）检测方法对其中一些粪便样本进行检测，结果表明10%的样本中存在微小隐孢子虫DNA。