Wang X, Brown I L, Khaled D, Mahoney M C, Evans A J, Conway P L
CRC Food Industry Innovation, School of Medicine, The University of Queensland, Mater Adult Hospital, South Bank, Australia.
J Appl Microbiol. 2002;93(3):390-7. doi: 10.1046/j.1365-2672.2002.01704.x.
To study the effects of amylomaize starch and modified (carboxymethylated and acetylated) amylomaize starches on the composition of colonic bacteria and the production of volatile fatty acids, in mice.
Balb/c mice were fed with experimental diets containing various amount of amylomaize and modified amylomaize starches. Colonic bacterial populations and short-chain fatty acids were monitored. Results showed that the increases in indigenous bifidobacteria were detected in mice fed all starches tested; however, the highest numbers were observed in the group fed with 40% unmodified amylomaize starch. The starch type influenced the populations of indigenous Lactobacillus, Bacteroides and coliforms. High Lactobacillus numbers were achieved in the colon of mice fed with high concentration of amylomaize starch. Acetylated amylomaize starch significantly reduced the population of coliforms. In addition, orally dosed amylomaize utilizing bifidobacteria reached their highest levels when fed together with amylomaize or carboxymethylated amylomaize starch and in both cases butyrate levels were markedly increased.
These results indicate that different amylomaize starches could generate desirable variation in gut microflora and that particular starches may be used to selectively modify gut function.
Amylomaize starch appeared to enhance the desirable composition of colonic bacteria in mice, and suggested it possessed the potential prebiotic properties. Therefore, resistant starch and its chemical derivatives may exert beneficial impacts to the human colon.
研究支链玉米淀粉以及改性（羧甲基化和乙酰化）支链玉米淀粉对小鼠结肠细菌组成和挥发性脂肪酸产生的影响。
给Balb/c小鼠喂食含有不同量支链玉米淀粉和改性支链玉米淀粉的实验性饮食。监测结肠细菌种群和短链脂肪酸。结果显示，在喂食所有测试淀粉的小鼠中均检测到原生双歧杆菌增加；然而，在喂食40%未改性支链玉米淀粉的组中观察到的数量最高。淀粉类型影响原生乳酸杆菌、拟杆菌和大肠菌群的种群数量。喂食高浓度支链玉米淀粉的小鼠结肠中乳酸杆菌数量较高。乙酰化支链玉米淀粉显著减少了大肠菌群的数量。此外，口服利用支链玉米淀粉的双歧杆菌在与支链玉米淀粉或羧甲基化支链玉米淀粉一起喂食时达到最高水平，并且在这两种情况下丁酸盐水平均显著增加。
这些结果表明，不同的支链玉米淀粉可在肠道微生物群中产生理想的变化，并且特定的淀粉可用于选择性地调节肠道功能。
支链玉米淀粉似乎能增强小鼠结肠细菌的理想组成，并表明其具有潜在的益生元特性。因此，抗性淀粉及其化学衍生物可能对人类结肠产生有益影响。
