Valentini Anna Maria, Renna Letizia, Armentano Raffele, Pirrelli Michele, Di Leo Alfredo, Gentile Mattia, Caruso Maria Lucia
Department of Pathology, IRCCS S De Bellis, Castellana Grotte (BA), Italy.
Anticancer Res. 2002 Jul-Aug;22(4):2083-8.
Mismatch repair (MMR) proteins (MSH2 and MLH1) deficiency is responsible for microsatellite instability (MSI) status. We evaluated p53 and beta-catenin expressions in colorectal cancer specimens with known microsatellite status, previously assessed by means of the polymerase chain reaction (PCR). We also analyzed the MMR proteins immunostaining and compared the results with those ascertained by PCR.
Twenty-five colorectal cancer patients were analyzed for immunohistochemical expression of p53, beta-catenin, MSH2 and MLH1 proteins.
The microsatellite status was only significantly correlated with p53 expression and MRR proteins pattern.
We demonstrated a significantly higher p53 expression in MSI colorectal specimens. The concordance rate between immunohistochemistry and PCR was so high (80%) that the immunohistochemical technique can be proposed as a method to select MSI patients for improved outcome and response to chemotherapy.
错配修复（MMR）蛋白（MSH2和MLH1）缺陷导致微卫星不稳定性（MSI）状态。我们评估了已知微卫星状态的结直肠癌标本中p53和β-连环蛋白的表达情况，此前通过聚合酶链反应（PCR）进行了评估。我们还分析了MMR蛋白的免疫染色，并将结果与通过PCR确定的结果进行了比较。
对25例结直肠癌患者进行p53、β-连环蛋白、MSH2和MLH1蛋白的免疫组化表达分析。
微卫星状态仅与p53表达和MRR蛋白模式显著相关。
我们证实在MSI结直肠癌标本中p53表达显著更高。免疫组化与PCR之间的一致性率很高（80%），以至于免疫组化技术可被提议作为一种选择MSI患者以改善预后和化疗反应的方法。
