Key L N, Boyle P R, Jaspars M
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen AB24 2TZ, UK.
Toxicon. 2002 Jun;40(6):677-83. doi: 10.1016/s0041-0101(01)00254-9.
Haemolytic activity against mammalian erythrocytes was detected in very low concentrations of saliva from the octopus Eledone cirrhosa. It was not caused by any previously identified component of this saliva. The activity was slightly inhibited by trypsin and more drastically by chymotrypsin. Locust bioassay was used to test HPLC fractions isolated from E. cirrhosa saliva. An LD(50) of 378.4 microg/g locust weight was calculated for whole saliva injected into the desert locust Schistocerca gregaria. This study was the first record of haemolytic activity and toxicity to insects in cephalopod saliva. Both haemolytic and insecticidal fractions could be separated using reversed phase HPLC.
在极低浓度的卷腕蛸唾液中检测到了对哺乳动物红细胞的溶血活性。它不是由该唾液中任何先前已鉴定的成分引起的。该活性受到胰蛋白酶的轻微抑制，而受到胰凝乳蛋白酶的抑制作用更为显著。采用蝗虫生物测定法对从卷腕蛸唾液中分离得到的高效液相色谱馏分进行检测。对于注射到沙漠蝗虫（沙漠飞蝗）体内的全唾液，计算得出半数致死剂量（LD50）为378.4微克/克蝗虫体重。本研究是头足类动物唾液中溶血活性和对昆虫毒性的首次记录。使用反相高效液相色谱法可以分离溶血和杀虫馏分。
