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胆碱脱氢酶与某些电子受体的反应。

The reaction of choline dehydrogenase with some electron acceptors.

作者信息

Barrett M C, Dawson A P

出版信息

Biochem J. 1975 Dec;151(3):677-83. doi: 10.1042/bj1510677.

DOI:10.1042/bj1510677
PMID:1218095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172417/
Abstract
  1. The choline dehydrogenase (EC 1.1.99.1) WAS SOLUBILIZED FROM ACETONE-DRIED POWDERS OF RAT LIVER MITOCHONDRIA BY TREATMENT WITH Naja naja venom. 2. The kinetics of the reaction of enzyme with phenazine methosulphate and ubiquinone-2 as electron acceptors were investigated. 3. With both electron acceptors the reaction mechanism appears to involve a free, modified-enzyme intermediate. 4. With some electron acceptors the maximum velocity of the reaction is independent of the nature of the acceptor. With phenazine methosulphate and ubiquinone-2 as acceptors the Km value for choline is also independent of the nature of the acceptor molecule. 5. The mechanism of the Triton X-100-solubilized enzyme is apparently the smae as that for the snake venom solubilized enzyme.
摘要
  1. 用眼镜蛇毒处理大鼠肝脏线粒体的丙酮干粉，可使胆碱脱氢酶（EC 1.1.99.1）溶解。2. 研究了该酶与作为电子受体的吩嗪硫酸甲酯和泛醌-2反应的动力学。3. 对于这两种电子受体，反应机制似乎涉及一个游离的、修饰酶中间体。4. 对于某些电子受体，反应的最大速度与受体的性质无关。以吩嗪硫酸甲酯和泛醌-2作为受体时，胆碱的Km值也与受体分子的性质无关。5. Triton X-100溶解的酶的机制显然与蛇毒溶解的酶相同。

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本文引用的文献

1
THE MOLAR EXTINCTION COEFFICIENT OF 2,6-DICHLOROPHENOL INDOPHENOL.2,6-二氯酚靛酚的摩尔消光系数
Biochim Biophys Acta. 1964 Apr 4;86:194-7. doi: 10.1016/0304-4165(64)90180-1.
2
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations.具有两种或更多种底物或产物的酶催化反应动力学。I. 命名法和速率方程。
Biochim Biophys Acta. 1963 Jan 8;67:104-37. doi: 10.1016/0006-3002(63)91800-6.
3
Intermediates in the catalytic action of lipoyl dehydrogenase (diaphorase).硫辛酰脱氢酶（黄递酶）催化作用的中间产物。
Biochem J. 1960 Nov;77(2):341-51. doi: 10.1042/bj0770341.
4
Studies on choline dehydrogenase. I. Extraction in soluble form, assay, and some properties of the enzyme.
J Biol Chem. 1959 Jun;234(6):1605-10.
5
Solubilization and assay of the insoluble alpha-glycero-phosphate dehydrogenase of animal tissues.
Biochim Biophys Acta. 1958 Sep;29(3):661-2. doi: 10.1016/0006-3002(58)90036-2.
6
Preparation and some properties of soluble choline dehydrogenase.
Biochim Biophys Acta. 1958 Aug;29(2):463-4. doi: 10.1016/0006-3002(58)90227-0.
7
Graphical determination of the dissociation constants for two-substrate enzyme systems.双底物酶系统解离常数的图形测定法。
Biochim Biophys Acta. 1957 Sep;25(3):575-8. doi: 10.1016/0006-3002(57)90529-2.
8
Studies on succinic dehydrogenase. I. Preparation and assay of the soluble dehydrogenase.琥珀酸脱氢酶的研究。I. 可溶性脱氢酶的制备及测定
J Biol Chem. 1956 Apr;219(2):963-75.
9
A micro biuret method for protein determination; determination of total protein in cerebrospinal fluid.一种用于蛋白质测定的微量双缩脲法；脑脊液中总蛋白的测定
Scand J Clin Lab Invest. 1953;5(3):218-22. doi: 10.3109/00365515309094189.
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Preparation of soluble choline dehydrogenase from liver mitochondria.从肝线粒体中制备可溶性胆碱脱氢酶。
J Biol Chem. 1953 Aug;203(2):899-906.