胰岛素样生长因子-I对兔关节软骨细胞培养的影响
[Effect of insulin-like growth factor-I on cultured articular chondrocytes of rabbits].
作者信息
Liu Gang, Hu Yun-yu, Ma Ping, Han Yi-sheng
机构信息
Department of Orthopedic Surgery, Xijing Hospital, 4th Military Medical University, Xi'an Shaanxi, P. R. China 710032.
出版信息
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2002 Jul;16(4):228-30.
OBJECTIVE
To investigate the influence of insulin-like growth factor-I (IGF-I) on biological characteristics of articular chondrocytes cultured in vitro of rabbits.
METHODS
Monolayer articular chondrocytes of 4-week old rabbits were cultured in medium with IGF-I, at the concentrations of 3, 10, 30, 100, and 300 ng/ml. The DNA content in cells and glucuronic acid content in matrix were detected on the 2nd, 4th, 6th days after culture.
RESULTS
The DNA content in cells and the glucuronic acid content in matrix in articular chondrocytes cultured in medium with IGF-I at concentrations of 3-300 ng/ml were all significantly higher than those in control group (P < 0.01), which reached the peak at the concentrations of 30-100 mg/ml on the 4th day.
CONCLUSION
IGF-I could obviously promote the proliferation of articular chondrocytes in vitro, and there exist time-dependent and dose-dependent effect.
目的
探讨胰岛素样生长因子-I（IGF-I）对兔体外培养关节软骨细胞生物学特性的影响。
方法
将4周龄兔的单层关节软骨细胞培养于含浓度为3、10、30、100和300 ng/ml IGF-I的培养基中。培养第2、4、6天检测细胞中的DNA含量和基质中的葡萄糖醛酸含量。
结果
培养于含3 - 300 ng/ml IGF-I培养基中的关节软骨细胞，其细胞中的DNA含量和基质中的葡萄糖醛酸含量均显著高于对照组（P < 0.01），在第4天浓度为30 - 100 mg/ml时达到峰值。
结论
IGF-I可明显促进体外关节软骨细胞的增殖，且存在时间依赖性和剂量依赖性效应。
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