Ma Jinfeng, Chen Xiaoliang, Wang Yingzhen, Wang Jixian, Huang Hongshi
Department of Orthopaedics, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China.
Zhonghua Wai Ke Za Zhi. 2002 Aug;40(8):600-3.
To investigate the effect of cartilage-derived growth factor (CDGF) on cultured rabbit chondrocytes, and the relation between CDGF, insulin-like growth factor-I (IGF-I), and proliferation and metabolism of chondrocytes.
CDGF was extracted from chicken xiphoid in our laboratory. Chondrocytes were isolated from 3-week-old New Zealand male rabbits, and cultured in Ham's F-12 medium containing 10% fetal calf serum. The chondrocyts of second generation were treated with CDGF and/or IGF-I of different concentrations. With chloramine T method and MTT method, we compared the content of hydroxyproline in Ham's F-12 medium and observed proliferation and energy synthesis of chondrocytes.
CDGF and IGF-I both stimulated the proliferation and synthesis of hydroxyproline of chondrocytes dose-dependently. The optimal concentration of CDGF was 16 ng/ml and 32 ng/ml respectively, and that of IGF-I was 30 ng/ml. There was obvious synergic effect between CDGF and IGF-I.
CDGF can stimulate the proliferation and collagen synthesis of chondrocytes and has synergistic effect with IGF-I.
研究软骨衍生生长因子（CDGF）对培养的兔软骨细胞的作用，以及CDGF、胰岛素样生长因子-I（IGF-I）与软骨细胞增殖和代谢之间的关系。
CDGF由本实验室从鸡剑突中提取。从3周龄新西兰雄性兔分离软骨细胞，在含10%胎牛血清的Ham's F-12培养基中培养。用不同浓度的CDGF和/或IGF-I处理第二代软骨细胞。采用氯胺T法和MTT法，比较Ham's F-12培养基中羟脯氨酸含量，观察软骨细胞的增殖和能量合成。
CDGF和IGF-I均能剂量依赖性地刺激软骨细胞增殖和羟脯氨酸合成。CDGF的最佳浓度分别为16 ng/ml和32 ng/ml，IGF-I的最佳浓度为30 ng/ml。CDGF与IGF-I之间存在明显的协同作用。
CDGF能刺激软骨细胞增殖和胶原合成，并与IGF-I具有协同作用。
