体内促性腺激素释放激素（GnRH）激动剂和GnRH拮抗剂对体外培养的人颗粒黄体细胞中由人绒毛膜促性腺激素（hCG）和胰岛素刺激产生孕酮的影响。
Effect of in vivo GnRH agonist and GnRH antagonist on hCG and insulin-stimulated progesterone production by human granulosa-lutein cells in vitro.
作者信息
Mitwally Mohamed F M, Casper Robert F
机构信息
Department of Obstetrics and Gynecology, Samuel Lunenfeld Research Institute and Mount Sinai Hospital, University of Toronto, Ontario, Canada.
出版信息
J Assist Reprod Genet. 2002 Aug;19(8):384-9. doi: 10.1023/a:1016398405248.
PURPOSE
To investigate hCG and insulin-stimulated progesterone (P) production by human granulosa-lutein cells (hGLC) in vitro.
METHODS
hGLCs were isolated from patients undergoing IVF-ET cycles in which GnRH agonist or GnRH antagonist was used to prevent a midcycle gonadotropin surge. The cells were cultured for 3 days, and then treated with hCG 0.5, 1, and 10 IU/I, and insulin 0.01, 0.1, and 1 microM in serum free conditions. In vitro P production was measured by enzyme immunoassay.
RESULTS
hCG stimulated P production by hGLCs from cycles in which GnRH antagonist was used, but a blunted response was seen in GnRH-agonist treated cycles. Insulin-stimulated P production was similar in cells from cycles in which GnRH-agonist or GnRH-antagonist treatment was used.
CONCLUSIONS
Because insulin and hCG may share common pathways beyond the level of receptor activation, we hypothesize that GnRH agonist, but not GnRH antagonist, may affect the expression and/or activation of LH receptors in the hGLCs.
目的
研究人颗粒黄体细胞（hGLC）在体外受hCG和胰岛素刺激后的孕酮（P）分泌情况。
方法
从接受体外受精-胚胎移植（IVF-ET）周期治疗的患者中分离hGLC，这些患者使用促性腺激素释放激素（GnRH）激动剂或GnRH拮抗剂来防止周期中期促性腺激素激增。细胞培养3天，然后在无血清条件下分别用0.5、1和10 IU/I的hCG以及0.01、0.1和1 μM的胰岛素进行处理。通过酶免疫测定法测量体外P分泌量。
结果
hCG刺激了使用GnRH拮抗剂周期的hGLC分泌P，但在使用GnRH激动剂治疗的周期中反应减弱。在使用GnRH激动剂或GnRH拮抗剂治疗周期的细胞中，胰岛素刺激的P分泌情况相似。
结论
由于胰岛素和hCG可能在受体激活水平之上共享共同途径，我们推测GnRH激动剂而非GnRH拮抗剂可能影响hGLC中促黄体生成素（LH）受体的表达和/或激活。
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