Zhang Jian-Xu, Sun Lixing, Zhang Zhi-Bin, Wang Zu-Wang, Chen Yi, Wang Rui
State Key Laboratory of Integrated Management of Pest Insects and Rodents in Agriculture, Institute of Zoology, Chinese Academy of Sciences, Beijing.
J Chem Ecol. 2002 Jun;28(6):1287-97. doi: 10.1023/a:1016246120479.
The volatile constituents in anal gland secretions of two sympatric Mustela species, the Siberian weasel (M. sibirica) and steppe polecat (M. eversmanni), were studied by the headspace technique, followed by gas chromatography-mass spectrometry (GC-MS) analysis. Nine sulfur-containing compounds were identified. They were 2,2-dimethylthietane, (Z)- or (E)-2,4-dimethylthietane, (E)-2,3-dimethylthietane, 2-ethylthietane, (E)-2-ethyl-3-methylthietane, (Z)-2-ethyl-3-methylthietane, 2-propylthietane, 3,3-dimethyl-1,2-dithiacyclopentane, and (Z)-3,4-dimethyl-2,2-dithiacyclopentane. Among them, (E)-2-ethyl-3- methylthietanes, (Z)-2-ethyl-3-methylthietanes, and (Z)-3,4-dimethyl-1,2-dithiacyclopentane were present in the polecat but not in the weasel. The predominant compound was 2,2-dimethylthietane in the weasel and (E)- or (Z)-2,4-dimethylthietane in the polecat. These differences were consistent between the two species, regardless of sex and age and, therefore, could possibly be used for species recognition. In the weasel, 2-ethylthietane was found only in the female, and the relative abundance of several compounds was significantly different between males and females. In the polecat, although no sex-specific volatile compounds were found, males and females differed in the relative abundance of several of the compounds. In both species, the relative abundance of some compounds varied with age. We conclude that these volatile compounds can be used to communicate information about species, sex, and age.
采用顶空技术，随后进行气相色谱 - 质谱联用（GC - MS）分析，对两种同域分布的鼬属动物——黄鼬（Mustela sibirica）和艾鼬（Mustela eversmanni）肛腺分泌物中的挥发性成分进行了研究。鉴定出9种含硫化合物。它们分别是2,2 - 二甲基硫杂环丙烷、（Z）- 或（E）- 2,4 - 二甲基硫杂环丙烷、（E）- 2,3 - 二甲基硫杂环丙烷、2 - 乙基硫杂环丙烷、（E）- 2 - 乙基 - 3 - 甲基硫杂环丙烷、（Z）- 2 - 乙基 - 3 - 甲基硫杂环丙烷、2 - 丙基硫杂环丙烷、3,3 - 二甲基 - 1,2 - 二硫杂环戊烷和（Z）- 3,4 - 二甲基 - 2,2 - 二硫杂环戊烷。其中，（E）- 2 - 乙基 - 3 - 甲基硫杂环丙烷、（Z）- 2 - 乙基 - 3 - 甲基硫杂环丙烷和（Z）- 3,4 - 二甲基 - 1,2 - 二硫杂环戊烷存在于艾鼬中，而黄鼬中没有。黄鼬中主要的化合物是2,2 - 二甲基硫杂环丙烷，艾鼬中主要的化合物是（E）- 或（Z）- 2,4 - 二甲基硫杂环丙烷。这两个物种之间的这些差异是一致的，无论性别和年龄如何，因此有可能用于物种识别。在黄鼬中，仅在雌性中发现了2 - 乙基硫杂环丙烷，并且几种化合物的相对丰度在雄性和雌性之间存在显著差异。在艾鼬中，虽然未发现性别特异性挥发性化合物，但雄性和雌性在几种化合物的相对丰度上存在差异。在这两个物种中，一些化合物的相对丰度随年龄而变化。我们得出结论，这些挥发性化合物可用于传递有关物种、性别和年龄的信息。
