Guiot S R, Tartakovsky B, Lanthier M, Lévesque M J, Manuel M F, Beaudet R, Greer C W, Villemur R
Biotechnology Research Institute, Montréal, Québec, Canada.
Water Sci Technol. 2002;45(10):35-41.
Anaerobic degradation of pentachlorophenol (PCP) is an example of a process that may benefit from enrichment or bioaugmentation. In one approach, enrichment acceleration was attempted by applying an on-line control-based selective stress strategy to a native anaerobic upflow sludge bed (UASB) system; this strategy linked PCP loading rate to methane production. As a result, the reactor biomass potential for PCP complete dechlorination reached a rate of 4 mg g(-1) volatile suspended solid (VSS) day(-1) within a period of 120 days. In another approach, a pure culture, Desulfitobacterium frappieri PCP-1, a strictly anaerobic Gram-positive bacterium, was used to augment the granular biomass of the UASB reactor. This also resulted in a specific degradation rate of 4 mg PCPg(-1) VSS day(-1); however, this potential was attained within 56 days. Fluorescent in situ hybridization (FISH) showed that the PCP-1 strain was able to rapidly attach to the granule and densely colonize the outer biofilm layer.
五氯苯酚（PCP）的厌氧降解是一个可能受益于富集或生物强化的过程实例。在一种方法中，通过对原生厌氧上流污泥床（UASB）系统应用基于在线控制的选择性应激策略来尝试加速富集；该策略将五氯苯酚的负荷率与甲烷产量联系起来。结果，在120天内，该反应器中五氯苯酚完全脱氯的生物质潜力达到了4 mg g(-1)挥发性悬浮固体（VSS）/天的速率。在另一种方法中，使用一种纯培养物，即弗氏脱硫肠状菌PCP-1（一种严格厌氧的革兰氏阳性细菌）来强化UASB反应器的颗粒生物质。这也导致了4 mg五氯苯酚/g(-1) VSS/天的特定降解速率；然而，这种潜力在56天内就实现了。荧光原位杂交（FISH）表明，PCP-1菌株能够迅速附着在颗粒上，并在外部生物膜层密集定殖。
