Onyshchenko O M, Kiprianova O A, Lysenko T H, Smirnov V V
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Mikrobiol Z. 2002 May-Jun;64(3):38-44.
Antagonistic properties of 41 strains of Alteromonas-like bacteria isolated from the Black Sea water and molluscs have been studied. Being grown on the rich medium "B" for marine bacteria, 21% of strains have shown high antagonistic activity against phytopathogenic fungi; 6% of strains inhibited the growth of Bacillus subtilis, Proteus vulgaris and Candida albicans. Spectrum of antagonistic activity was essentially changed on synthetic "BM" medium with acetate, glutamate, alpha-alanine as a single source of carbon and was directed against Pseudomonas aeruginosa. Culture liquids and acetone extracts of microbial biomass of 34% of the studied strains have shown activity against bacteria, fungi and cyanobacteria. Strains producing the wide spectrum of antimicrobial substances (Alteromonas macleodii, Pseudoalteromonas citrea, P. haloplanktis, P. aurantia, Pseudoalteromonas sp.), fungicidal and algocidal substances have been found. Both extra- and intracellular metabolities of marine bacteria (including the pigments) were active.
对从黑海海水和软体动物中分离出的41株类交替单胞菌的拮抗特性进行了研究。在用于海洋细菌的丰富培养基“B”上生长时,21%的菌株对植物病原真菌表现出高拮抗活性;6%的菌株抑制枯草芽孢杆菌、普通变形杆菌和白色念珠菌的生长。在以乙酸盐、谷氨酸、α-丙氨酸作为单一碳源的合成“BM”培养基上,拮抗活性谱发生了本质变化,且针对铜绿假单胞菌。34%的研究菌株的培养液和微生物生物质的丙酮提取物对细菌、真菌和蓝细菌表现出活性。已发现产生广谱抗菌物质(麦克劳德交替单胞菌、柠檬假交替单胞菌、嗜盐假交替单胞菌、橙色假交替单胞菌、假交替单胞菌属)、杀真菌和杀藻物质的菌株。海洋细菌的胞外和胞内代谢产物(包括色素)均具有活性。