Antipchuk A F, Rangelova V N, Tantsiurenko E V
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Mikrobiol Z. 2002 May-Jun;64(3):44-51.
The species and strain sensitivity of free and symbiotic nitrogen-fixing agents to heavy metals: cadmium, copper, lead and zinc in the gradient of concentration from 0.5 to 20.0 MPC/l has been studied under the laboratory conditions. Azotobacter (35 strains) and rhizobia (63 strains) have been tested. It has been established that zinc and copper are more toxical for free nitrogen-fixing agents than lead and cadmium. Among symbiotrophic nitrogen-fixing agents the nodule bacteria for alfalfa were most sensitive to cadmium; nodule bacteria of pea and lupine--to copper; those of galega and soya--to zinc. Lead proved to be the least toxic for rhizobia. Negative effect of the heavy-metal mix on diasotrophs was higher than that of each separate metal. The cultures resistant to the effect of heavy metals have been found which may be used when producing preparation for biological reclamation of polluted soils.
在实验室条件下，研究了游离和共生固氮剂对重金属（镉、铜、铅和锌）在浓度从0.5到20.0 MPC/升梯度下的物种和菌株敏感性。对35株固氮菌和63株根瘤菌进行了测试。已确定锌和铜对游离固氮剂的毒性比对铅和镉更大。在共生固氮剂中，苜蓿根瘤菌对镉最敏感；豌豆和羽扇豆根瘤菌对铜敏感；山羊豆和大豆根瘤菌对锌敏感。事实证明，铅对根瘤菌的毒性最小。重金属混合物对固氮菌的负面影响高于每种单独金属的影响。已发现对重金属影响具有抗性的培养物，可用于生产污染土壤生物修复制剂。
