Pyroh T P, Hrinberh T O, Malashenko Iu R
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Mikrobiol Z. 2002 May-Jun;64(3):81-94.
Basing on the development of approaches to the controlled regulation of synthesis of the complex polysaccharide preparation ethapolan (producer Acinetobacter sp.) the strategy of obtaining microbial exopolysaccharides (EPS) with stable composition and properties was determined. The strategy is based on the following principles of regulation of composition, physico-chemical properties and synthesis intensification of EPS: 1) to find out EPS functional groups determining their physico-chemical properties and factor providing synthesis of EPS with certain functional groups; 2) to study changes of EPS composition and properties during producer cultivation and to determine growth phase in which the synthesis of EPS possessing necessary properties occurs; 3) to investigate interrelation between EPS physico-chemical properties and their protective functions and to determine cultivation conditions necessary for development of EPS protective functions; 4) analysis of metabolic pathways of EPS synthesis, elucidation of "bottlenecks" in producer's metabolism and search for ways toward their elimination.
基于对复杂多糖制剂乙磺半乳聚糖（生产者为不动杆菌属）合成的可控调节方法的发展，确定了获得具有稳定组成和性质的微生物胞外多糖（EPS）的策略。该策略基于以下调节EPS组成、物理化学性质和合成强化的原则：1）找出决定其物理化学性质的EPS官能团以及提供具有特定官能团的EPS合成的因素；2）研究生产者培养过程中EPS组成和性质的变化，并确定产生具有所需性质的EPS的生长阶段；3）研究EPS物理化学性质与其保护功能之间的相互关系，并确定EPS保护功能发展所需的培养条件；4）分析EPS合成的代谢途径，阐明生产者代谢中的“瓶颈”并寻找消除它们的方法。
