Cui G L, Sandvik A K, Munkvold B, Waldum H L
Dept of Intra-abdominal Diseases, Norwegian University of Science and Technology, Faculty of Medicine, University Hospital of Trondheim.
Scand J Gastroenterol. 2002 Jul;37(7):750-3. doi: 10.1080/00365520213249.
Anaesthetic agents affect gastric acid secretion, but the mechanisms behind this action have not been fully evaluated. The enterochromaffin-like (ECL) cell plays a key role in the regulation of gastric acid secretion, and anaesthetic agents have recently been described as inhibiting histamine release from the ECL cell. The present study examines the effect of anaesthetic agents on the ECL cell and on parietal cell functions.
Different concentrations of urethane, pentobarbital and a mixture of fluanisone/fantanyl/midazolam (FFM) were examined for the effect on gastrin-stimulated histamine release and acid secretion and on histamine-stimulated acid secretion in the totally isolated vascularly perfused rat stomach. The luminal acid output and histamine concentrations in venous effluents were measured by titration and radioimmunoassay, respectively.
Pentobarbital caused an inhibition on both histamine release and acid output in gastrin-stimulated stomachs in a concentration-dependent way. The mixture of FFM at higher concentrations inhibited histamine release from the ECL cell and luminal H+ output in gastrin-stimulated acid secretion. Urethane exerted a slight inhibitory effect on histamine release only at the lowest concentration. Pentobarbital also reduced histamine-stimulated gastric acid secretion, while the mixture of FFM did not.
pentobarbital inhibits acid secretion both by reducing ECL cell histamine release and parietal cell H+ secretion, whereas FFM inhibits acid secretion by interaction with the ECL cell only. Urethane also had a slight inhibitory effect on the ECL cell histamine release at the lowest concentration.
麻醉剂会影响胃酸分泌，但其作用背后的机制尚未得到充分评估。肠嗜铬样（ECL）细胞在胃酸分泌调节中起关键作用，最近有研究表明麻醉剂可抑制ECL细胞释放组胺。本研究旨在探讨麻醉剂对ECL细胞和壁细胞功能的影响。
在完全隔离并经血管灌注的大鼠胃中，检测不同浓度的乌拉坦、戊巴比妥以及氟胺酮/芬太尼/咪达唑仑混合物（FFM）对胃泌素刺激的组胺释放、酸分泌以及组胺刺激的酸分泌的影响。分别通过滴定法和放射免疫分析法测定管腔酸输出和静脉流出液中的组胺浓度。
戊巴比妥以浓度依赖的方式抑制胃泌素刺激的胃中组胺释放和酸输出。较高浓度的FFM混合物抑制胃泌素刺激的酸分泌中ECL细胞的组胺释放和管腔H⁺输出。乌拉坦仅在最低浓度时对组胺释放有轻微抑制作用。戊巴比妥还降低了组胺刺激的胃酸分泌，而FFM混合物则没有。
戊巴比妥通过减少ECL细胞组胺释放和壁细胞H⁺分泌来抑制酸分泌，而FFM仅通过与ECL细胞相互作用来抑制酸分泌。乌拉坦在最低浓度时对ECL细胞组胺释放也有轻微抑制作用。
