Sobhon P
Acta Anat (Basel). 1979;105(4):494-504. doi: 10.1159/000145156.
Embedding kidney in polymerized glutaraldehyde-urea favors the retention of glycoprotein matrix of the cell coat and the basement membrane of the glomeruli. The basement membrane appears as a single layer with uniform amorphous matrix. Thick glycoprotein coat covers the whole surface of prodocytes and their foot processes. In areas other than the slits and the portion of the foot processes which touch on the basement membrane, the coat is a continuous layer with an average thickness of 490 A. In the slits between the foot processes of podocytes there is an actual fusion of glycoprotein coats; the average width of the slit is 415 A. The glycoprotein 'plugs' in the slit may be a significant portion of the glomerular filtration barrier against macromolecules, together with the basement membrane and the slit diaphragms.
将肾脏包埋于聚合的戊二醛 - 尿素中有利于保留细胞被膜的糖蛋白基质和肾小球的基底膜。基底膜呈现为具有均匀无定形基质的单层结构。厚厚的糖蛋白被膜覆盖足细胞及其足突的整个表面。在裂隙以及足突与基底膜接触部分以外的区域，被膜是一层连续的结构，平均厚度为490埃。在足细胞足突之间的裂隙处，糖蛋白被膜实际发生融合；裂隙的平均宽度为415埃。裂隙中的糖蛋白“栓子”可能是肾小球对大分子滤过屏障的重要组成部分，与基底膜和裂隙隔膜共同起作用。
