Ovigne J-M, Baker B S, Davison S C, Powles A V, Fry L
Dermatology Research, Faculty of Medicine, Imperial College of Science Technology and Medicine, St. Mary's campus, London, UK.
Exp Dermatol. 2002 Aug;11(4):357-64. doi: 10.1034/j.1600-0625.2002.110410.x.
Chronic plaque psoriasis is a T cell mediated disease associated with group A streptococci (GAS). We have previously shown the presence of a psoriasis-specific dermal Th1 subset that recognizes GAS antigens. To assess whether GAS-reactive T cells are also present in lesional epidermis, fresh cell suspensions or T cell lines isolated from lesional epidermis of 33 psoriasis patients were stained for intracellular interferon-gamma after stimulation with GAS antigens. The patients were typed by PCR for HLA-DR7 and HLA-Cw6 expression. A subset of GAS-reactive CD8+ T cells (2.4% +/- 2.4) was found in 14/21 (67%) fresh cell suspensions. A smaller subset of GAS-reactive CD4+ T cells (0.9% +/- 0.9) was found in 13/21 (62%) fresh cell suspensions, which was expanded in the T cell lines. There was a significant inverse correlation between the proportions of GAS-reactive CD4+ and CD8+ T cells in the fresh suspensions (r = -0.48, P = 0.0277). The presence of GAS-reactive CD4+ or CD8+ T cells did not correlate with HLA-DR7 or HLA-Cw6 expression, respectively. This study has demonstrated GAS-reactive CD8+, and to a lesser extent CD4+, T cell subsets in psoriatic epidermis and provides further evidence that GAS antigens may play a role in the pathogenesis of chronic plaque psoriasis.
慢性斑块状银屑病是一种与A组链球菌（GAS）相关的T细胞介导的疾病。我们之前已经证明存在一种识别GAS抗原的银屑病特异性真皮Th1亚群。为了评估GAS反应性T细胞是否也存在于皮损表皮中，对从33例银屑病患者的皮损表皮中分离出的新鲜细胞悬液或T细胞系用GAS抗原刺激后进行细胞内干扰素-γ染色。通过PCR对患者进行HLA-DR7和HLA-Cw6表达分型。在14/21（67%）的新鲜细胞悬液中发现了一部分GAS反应性CD8+ T细胞（2.4%±2.4）。在13/21（62%）的新鲜细胞悬液中发现了比例较小的GAS反应性CD4+ T细胞（0.9%±0.9），其在T细胞系中有所扩增。新鲜悬液中GAS反应性CD4+和CD8+ T细胞的比例之间存在显著的负相关（r = -0.48，P = 0.0277）。GAS反应性CD4+或CD8+ T细胞的存在分别与HLA-DR7或HLA-Cw6表达无关。这项研究证明了银屑病表皮中存在GAS反应性CD8+ T细胞亚群，以及程度较轻的CD4+ T细胞亚群，并进一步证明GAS抗原可能在慢性斑块状银屑病的发病机制中起作用。
