Guo Fei, Lu Roujian, Sun Zhaohui, Ma Hailun, Li Jun, Zhang Yingmei, Ma Dalong, Ruan Li
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2002 Jun;16(2):136-41.
To investigate expression of IL-6 in non?replicating vaccinia virus and its immune effects on recombinant virus.
The recombinant non replicating vaccinia virus RVJ123 delta CK11 beta 75IL6 was constructed with non?replicating vaccinia virus vector pNEOCK11beta75IL6 and replicating vaccinia virus RVJ123. In animal model, immunization with the recombinant virus was carried out and its immune response was studied.
The recombinant virus could express IL-nd HBsAg simultaneously. Southern blot analysis demonstrated that the genes between vaccinia virus Hind? C and K fragments were deleted and IL-6 gene was integrated stably. Given intranasal inocula of the virus to immunize BALB/c mouse and New Zealand Rabbit, the elevated anti-HBsAg IgA and IgG antibody secreting cells in mouse lung lymphoid to vectors expressing IL-6 was at about two?fold higher level than those elicited by control virus at day 14 after immunization. Authors also could detect elevated anti-HBsAg IgA and IgG antibody conversion in mouse serum and lung fluid, rabbits serum, lung fluid, saliva, vagina and nasal washing samples.
IL-6 expressed by non-replicating recombinant vaccinia virus could enhance the induced?immune effects, it could serve as the effective adjuvant for recombinant vector vaccine.
研究白细胞介素-6（IL-6）在非复制型痘苗病毒中的表达及其对重组病毒的免疫作用。
用非复制型痘苗病毒载体pNEOCK11beta75IL6和复制型痘苗病毒RVJ123构建重组非复制型痘苗病毒RVJ123 delta CK11 beta 75IL6。在动物模型中，用重组病毒进行免疫并研究其免疫反应。
重组病毒能同时表达IL-6和乙肝表面抗原（HBsAg）。Southern印迹分析表明痘苗病毒HindⅢ C和K片段之间的基因被删除，IL-6基因稳定整合。经鼻接种该病毒免疫BALB/c小鼠和新西兰兔，免疫后第14天，小鼠肺淋巴组织中分泌抗HBsAg IgA和IgG抗体的细胞水平比对照病毒诱导的水平高约两倍。作者还能在小鼠血清和肺液、兔血清、肺液、唾液、阴道和鼻腔冲洗样本中检测到抗HBsAg IgA和IgG抗体转化率升高。
非复制型重组痘苗病毒表达的IL-6可增强诱导的免疫效果，可作为重组载体疫苗的有效佐剂。
