[采用SYBR Green 1实时聚合酶链反应检测乙肝病毒DNA]
[Detection of the HBV DNA with Sybr green 1 realtime polymerase chain reaction].
作者信息
Wang Yan, Xu Xiaoyuan, He Wei, Liu Zhihong, Gong Weibo, Wang Qinhuan
机构信息
Department of Infectious Diseases, First Hospital of Beijing University, Beijing 100034, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2002 Jun;16(2):160-1.
BACKGROUND
To search a novel sensitive, specific and lower cost method applicable for quantitative analysis of the hepatitis B virus DNA extensively.
METHODS
Quantitative analysis of the DNA from 100 sera by real-time PCR with Sybr green 1. The results of Sybr's assay were compared with the results obtained with Taqman's fluorescent quantitative assay.
RESULTS
Taqman real-time PCR could help evaluate the level of virus reliably. The results of Sybr's assay were in agreement with the Taqman's assay, but detection rate was lower.
CONCLUSIONS
Sybr green 1 real-time PCR appeared to be convenient and cheap, but detection rate was lower.
背景
广泛寻找一种适用于乙肝病毒DNA定量分析的新型灵敏、特异且低成本的方法。
方法
采用Sybr green 1实时荧光定量PCR对100份血清中的DNA进行定量分析。将Sybr法的检测结果与Taqman荧光定量法的结果进行比较。
结果
Taqman实时荧光定量PCR能够可靠地评估病毒水平。Sybr法的检测结果与Taqman法一致，但检测率较低。
结论
Sybr green 1实时荧光定量PCR方法简便、成本低，但检测率较低。
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