Huang Bin, Li Bojun, Zhou Weimin, Pi Guohua, Tang Weiping, Gu Shuyan
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2002 Jun;16(2):184-6.
To find a rapid and sensitive method for early diagnosis of nasopharyngeal carcinoma by using EBV TK kinase.
Prokaryotic expression plasmid pRSETTK was constructed. EBV TK kinase was highly expressed in E.coil BL21 (DE3). The authors identified specificity of TK kinase by Western blot, then used purified TK kinase in ELISA to detect the IgG antibody in the serum of NPC patients.
Specific IgG antibody against TK kinase was found in the serum of NPC patients. The specificity and sensitivity of TK kinase were both 100% in Western blot and were 98.0% and 93.4% respectively in ELISA.
The EBV TK kinase showed high specificity and sensitivity in ELISA, therefore it can be used for early diagnosis of NPC
通过使用EBV TK激酶寻找一种快速且灵敏的鼻咽癌早期诊断方法。
构建原核表达质粒pRSETTK。EBV TK激酶在大肠杆菌BL21(DE3)中高表达。作者通过蛋白质免疫印迹法鉴定TK激酶的特异性，然后在酶联免疫吸附测定法中使用纯化的TK激酶检测鼻咽癌患者血清中的IgG抗体。
在鼻咽癌患者血清中发现了针对TK激酶的特异性IgG抗体。TK激酶在蛋白质免疫印迹法中的特异性和灵敏度均为100%，在酶联免疫吸附测定法中分别为98.0%和93.4%。
EBV TK激酶在酶联免疫吸附测定法中显示出高特异性和灵敏度，因此可用于鼻咽癌的早期诊断。
