Naito T, Kawamura T, Bannai M, Kosaka T, Kameyama H, Shimamura K, Hoshi O, Ushiki T, Hatakeyama K, Abo T
Department of Immunology, Niigata University School of Medicine, Japan.
Clin Exp Immunol. 2002 Sep;129(3):397-404. doi: 10.1046/j.1365-2249.2002.01910.x.
Denatured syngeneic liver tissue prepared by mechanical procedures was intraperitoneally injected into adult C57BL/6 mice. In parallel with a decrease in the total number of lymphocytes in the liver, spleen, and thymus from days 1-7 after the injection, the proportion of the CD4+NK1.1+CD3(int) subset of these cells (i.e. natural killer T or NKT cells) increased in the liver. Even the absolute number of these NKT cells increased in the liver on days 14 and 21. In response to the injection of denatured liver tissue, tissue damage was induced in the liver, as shown by elevated levels of serum transaminases and hepatocyte degeneration observed by electron microscopy. Sera obtained on days 7 and 14 contained autoantibodies including anti-DNA antibodies. The proportion of CD1d(high)B cells in the liver was found to decrease on days 1-7. In other words, denatured liver tissue stimulated both NKT cells and certain B cells in the liver. These results suggest that liver lymphocytes might contain not only autoreactive T cells (e.g. CD3(int) or NKT cells) but also some B cells (e.g. B-1 cells) which produce autoantibodies and that the denatured tissue had the potential to stimulate these lymphocytes and to evoke an autoimmune-like state.
通过机械方法制备的变性同基因肝组织被腹腔注射到成年C57BL/6小鼠体内。在注射后第1至7天，肝脏、脾脏和胸腺中的淋巴细胞总数减少的同时，这些细胞中CD4+NK1.1+CD3(int)亚群（即自然杀伤T细胞或NKT细胞）在肝脏中的比例增加。甚至在第14天和21天，肝脏中这些NKT细胞的绝对数量也增加了。注射变性肝组织后，肝脏出现组织损伤，血清转氨酶水平升高以及电子显微镜观察到的肝细胞变性表明了这一点。在第7天和第14天获得的血清中含有包括抗DNA抗体在内的自身抗体。发现肝脏中CD1d(high)B细胞的比例在第1至7天下降。换句话说，变性肝组织刺激了肝脏中的NKT细胞和某些B细胞。这些结果表明，肝脏淋巴细胞可能不仅包含自身反应性T细胞（如CD3(int)或NKT细胞），还包含一些产生自身抗体的B细胞（如B-1细胞），并且变性组织有刺激这些淋巴细胞并引发类似自身免疫状态的潜力。
